Experiment context

Reader notes supplied by the result owner to help interpret this Covary run.

Experiment or dataset title

240 synthetic Y-STR-seq profiles for human kinship and species classification

Objectives

To test if Covary can run a simultaneous, parallel, multi-species Y-STR-based kinship and species separation analysis.

Samples / sequences

>DYS19_Brother_B1 TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS19_Chimp TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS19_Founder_F0 TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS19_Gorilla TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS19_Macaque TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS19_PaternalCousin_C1 TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS19_Son_S1 TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS19_Son_S2 TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS19_Unrelated_U1 TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS19_Unrelated_U2 TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGA >DYS385A_Brother_B1 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385A_Chimp GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385A_Founder_F0 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385A_Gorilla GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385A_Macaque GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385A_PaternalCousin_C1 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385A_Son_S1 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385A_Son_S2 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385A_Unrelated_U1 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385A_Unrelated_U2 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_Brother_B1 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_Chimp GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_Founder_F0 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_Gorilla GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_Macaque GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_PaternalCousin_C1 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_Son_S1 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_Son_S2 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_Unrelated_U1 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS385B_Unrelated_U2 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA >DYS388_Brother_B1 GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS388_Chimp GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS388_Founder_F0 GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS388_Gorilla GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS388_Macaque GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS388_PaternalCousin_C1 GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS388_Son_S1 GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS388_Son_S2 GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS388_Unrelated_U1 GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS388_Unrelated_U2 GATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA >DYS389I_Brother_B1 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389I_Chimp TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389I_Founder_F0 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389I_Gorilla TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389I_Macaque TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389I_PaternalCousin_C1 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389I_Son_S1 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389I_Son_S2 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389I_Unrelated_U1 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389I_Unrelated_U2 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_Brother_B1 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_Chimp TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_Founder_F0 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_Gorilla TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_Macaque TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_PaternalCousin_C1 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_Son_S1 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_Son_S2 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_Unrelated_U1 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS389II_Unrelated_U2 TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG TCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTG >DYS390_Brother_B1 TCTGTCTGTCTGTCT

Interpretation notes

There are remarkable establishment of possible kinship and species delineation. Coalescence analysis showed that: 1. STRs from unrelated individuals clustered together away from the actual kinship lineage. 2. Brothers (son1 and son2) linked together with close relationship between the cousin and father. 3. Chimp, gorilla, and macaque sequences formed distinct clusters, distant from human STRs. Note: the sequences used are synthetic and for exploratory only to see if Covary renders expected results.